Invitrogen™ Caspase 3 (Cleaved) Human ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ KHO1091

Catalog No. KHO1091

Includes: Caspase-3 Antibody-Coated 96-Well Plate; The Caspase-3 (Active) Standard; Standard Diluent Buffer; The Caspase-3 (Active) Detection Antibody; anti-rabbit IgG-HRP (100X); HRP Diluent; Wash Buffer Concentrate (25X); Stabilized Chromogen, TMB; Stop Solution; Plate Covers; Detailed protocol with validation tests

Description

The Human Caspase 3 (Cleaved) ELISA Kit is designed to detect and quantify the level of Caspase 3 (Cleaved) in fresh or frozen human cell lysates. The assay recognizes both natural and recombinant Caspase 3 (Cleaved). Principle of the method A monoclonal capture antibody specific for Caspase 3 (Cleaved) has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits,large and small,that dimerize to form the active enzyme. This protein cleaves and activates caspases 6,7 and 9,and the protein itself is processed by caspases 8,9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein,which is associated with neuronal death in Alzheimer′s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Order Info

Shipping condition: Wet ice

Specifications

• Accession Number: P42574
• Assay Sensitivity: 0.033 ng/mL
• Conjugate: Biotin
• Sample Type: Cell Lysates
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CPP32,CPP32B,SCA-1
• Interassay CV: 7.4%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Marker Gene: Caspase-3
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Assay Range: 0.039-2.5 ng/mL
• Concentration: 0.039 to 2.5ng/mL
• Product Type: ELISA Kit
• Target Species: Human
• Gene ID (Entrez): 836
• Incubation Time: 4 hr.
• Intraassay CV: 4.2%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Cell Lysate,10 μL
• Target: Human
• Total Assay Time: 4 hr.

For Research Use Only. Not for use in diagnostic procedures.