Invitrogen™ Tau (Phospho) [pS199] Mouse ELISA Kit

Sandwich ELISA Kit

Supplier:  Invitrogen™ KMB7041

Catalog No. KMB7041

Includes:

Two vials Ms Tau [pS199] Standard; 25mL Standard Diluent Buffer; 1 plate (12 x 8 well strips), antibody-coated wells; 11mL Ms Tau (pS199) Detection Antibody; 0.125mL Anti-rabbit IgG HRP (100X); 25mL HRP Diluent; 100mL Wash Buffer Concentrate (25X); 25mL Stabilized Chromogen, Tetramethylbenzidine; 25mL Stop Solution; 3 Plate Covers

Description

The Mouse Tau (Phospho) [pS199] ELISA quantitates Ms Tau (Phospho) [pS199] in mouse cell lysates, cell culture medium and brand homogenates. The assay will exclusively recognize both natural and recombinant Ms Tau (Phospho) [pS199]. Principle of the method The Ms Tau (Phospho) [pS199] solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Order Info

Shipping condition: Wet ice

Specifications

• Accession Number: P10637
• Assay Sensitivity: <58.5 pg/mL
• Conjugate: Biotin
• Sample Type: Brain Homegenate, Cell Lysates, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Incubation Time: 4 hr.
• Intraassay CV: 5.6%
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Brain Homogenate,10 μL; Cell Lysate,5 μL; Supernatant, 50 μL
• Target: Mouse
• Total Assay Time: 4 hr.

• Assay Range: 313-20,000 pg/mL
• Concentration: 15.6 to 1000pg/mL
• Product Type: ELISA Kit
• Target Species: Mouse
• Gene Symbol: AI413597,AW045860,Mtapt,Tau
• Interassay CV: 3.8%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Marker Gene: Tau
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

For Research Use Only. Not for use in diagnostic procedures.