Invitrogen™ Human Tau (Total) ELISA Kit

Description

Includes

Pre-coated plate, standard, biotinylated detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

ELISA

The Human Tau (Total) ELISA quantitates Hu Tau in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Tau, independent of phosphorylation state. Principle of the method The Human Tau solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

Order Info

Shipping condition: Wet ice

Specifications

• Accession Number: P10636
• Assay Range: 31 to 2000 pg/mL
• Assay Sensitivity: <10 pg/mL
• Concentration: 31 to 2000 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Cerebrospinal Fluid, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 4137
• Gene Symbol: DDPAC,FTDP-17 L,MSTD,MTBT1,MTBT2,PPND,PPP1R103,TAU,MAPT
• Incubation Time: 4 hr.
• Interassay CV: 6.5%
• Intraassay CV: 4.6
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Marker Gene: Tau
• Quantity: 2 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cerebrospinal Fluid 50 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Target: Tau
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs

For Research Use Only. Not for use in diagnostic procedures.