Invitrogen™ Livestock SAA ELISA Kit

Description

ELISA

The Livestock SAA ELISA Kit quantifies equine, porcine, feline, bovine and canine Serum Amyloid A (SAA) in serum and plasma. The assay will recognize both the natural and recombinant SAA. Principle of the method The Livestock SAA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The serum amyloid A family comprises a number of differentially expressed apolipoproteins, acute-phase SAA1 and SAA2, the former being the major component in plasma and constitutive SAAs. Although the liver is the primary site of synthesis of both SAA types extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed its importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations; SAA rises earlier and more sharply than CRP. Recently, a broader view of SAA expression and function has been emerging.

Specifications

• Accession Number: P19708,P19857,P35541
• Assay Range: 9.4 to 150 μg/mL
• Assay Sensitivity: 1.5 μ g/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum
• Target Species: Canine, Equine, Porcine, Bovine
• For Use With (Application): Colorimetric Detection
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 100033905, 100034017, 100525856, 403585, 480749, 616035, 751814
• Gene Symbol: PIG4,SAA,SAA2,TP53I4
• Incubation Time: 1.25 hr.
• Interassay CV: 11.7%
• Intraassay CV: 5.9
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, HRP conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution
• Label or Dye: HRP
• Marker Gene: SAA
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 1 μL, Serum 1 μL
• Storage Requirements: 2°C to 8°C
• Target: SAA
• Test Time: 1 hrs 20 mins
• Total Assay Time: 1 hrs 15 mins