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Invitrogen™ Novex™ TBE-Urea Sample Buffer (2X)
Description
For optimum performance, Novex™ TBE-Urea Sample Buffer is recommended for use with Novex™ TBE-urea gels.
- Contains urea and density agent Ficoll™
- Yields sharper, straighter bands than conventional density agents, plus tracking dyes bromophenol blue and xylene cyanol
Acrylamide Gel Electrophoresis, DNA & RNA Purification & Analysis, Nucleic Acid Gel Electrophoresis & Blotting
Order Info
Shipping Condition: Wet Ice
Specifications
Specifications
| Buffer | Sample Loading Buffer |
| Concentration | 2 X |
| Content And Storage | Store at 2°C to 8°C |
| Gel Compatibility | Polyacrylamide Gels, Agarose Gels |
| For Use With (Application) | Nucleic Acid Gel Electrophoresis, Blotting |
| Label or Dye | Bromophenol Blue, Xylene Cyanol |
| Product Line | Novex |
| Product Type | TBE Sample Buffer |
| Quantity | 10 mL |
| Shipping Condition | Wet Ice |
Frequently Asked Questions (FAQs)
If a slight turbidity develops, the fine precipitate can be dissolved by autoclaving for 5 minutes at 110°C. Do not autoclave in the container supplied. This treatment has no deleterious effect on the buffering properties of TBE.
There are many sample buffer formulations used, however we have found a distinct difference in the band appearance depending on the sample buffer composition. After evaluating urea, formamide, and various buffer systems, we found that the sharpest, flattest bands were obtained with a urea, Ficoll, and TBE buffer solution. Sample buffers made with formamide resulted in fuzzy, indistinct bands.
(1) Ethidium bromide: Soak gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 min. Destain by rinsing with three successive 10-min rinses of ultrapure water. Visualize bands under UV light. The gel must be removed from the cassette prior to visualization of the DNA under a UV light. Because polyacrylamide quenches the fluorescence of ethidium bromide, it is not possible to detect bands that contain less than about 10 ng of DNA by this method. SilverXpress and SYBR stains will provide greater detection sensitivity.
(2) Invitrogen SilverXpress Stain: Follow the standard procedure from the instruction booklet for staining TBE or TBE-Urea gels.
(3) SYBR Green I/II Nucleic Acid Gel Stain: See the SYBR Green Staining Manual for protocol details.
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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