Invitrogen™ Human TNF alpha ELISA Kit

ELISA

Supplier:  Invitrogen™ KHC3011

Catalog No. KHC3011

Includes: Human TNF-α Antibody Coated 96-Well Plate
Human TNF-α Standard
Standard Diluent Buffer
Human TNF-α Detection Antibody
Streptavidin-HRP
HRP Diluent
Wash Buffer Concentrate (25X)
Stabilized Chromogen, TMB
Stop Solution
Plate Covers
etailed protocol with validation tests

Description

The Human Tumor Necrosis Factor alpha (Hu TNFα) ELISA quantitates Hu TNFα in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TNFα. Principle of the method The Human TNFα solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Order Info

Shipping Condition: Dry Ice

Specifications

• Accession Number: P01375
• Assay Sensitivity: 1.7 pg/mL
• Product Type: ELISA
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: DIF-alpha,TNFA,TNFSF2,TNLG1F,TNF
• Interassay CV: 7.5%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Marker Gene: TNF-α
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hrs 20 mins

• Assay Range: 15.6 to 1000 pg/mL
• Conjugate: HRP
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Application): Colorimetric Detection
• Gene ID (Entrez): 7124
• Incubation Time: 4 hr.
• Intraassay CV: 4.4
• Label or Dye: HRP
• Quantity: 96 Tests
• Sample Volume: Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
• Target: TNF alpha
• Total Assay Time: 4 hrs

For Research Use Only. Not for use in diagnostic procedures.