Invitrogen™ Novex™ Tricine SDS Sample Buffer (2X)

Catalog No.	Quantity
LC1676	20 mL

Catalog No. LC1676 Supplier Invitrogen™ Supplier No. LC1676

Description

Use with Novex Tricine Gels

In the tricine system developed by Schaegger and von Jagow (1), tricine replaces glycine in the running buffer. This results in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.

1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Wet Ice

Specifications

• Chemical Name or Material: Sample Loading Buffer
• Concentration: 2X
• Physical Form: Liquid
• Product Line: Novex
• Quantity: 20 mL

Frequently Asked Questions (FAQs)

Can urea be used with the precast Tricine gel electrophoresis system to achieve denatured results?

Adding urea to the sample and running buffers, in conjunction with SDS, may provide improved solubilization of the sample if denaturation by SDS does not prove to be sufficient. This must be tested empirically for the protein of interest.

How long should I run the Novex Tricine Gels (e.g. Cat. No. EC6675BOX) and how do I recognize the running front?

You should run the gel until the phenol red tracking dye from the Novex Tricine SDS Sample Buffer (Cat. No. LC1676) reaches the bottom of the gel. Phenol red serves as an indicator of the running front as it is a very small molecule that migrates with the ion front in Tricine gels. The Coomassie from the sample buffer runs a little slower and can be 1-2 cm behind the phenol red.

Does the Novex Tricine SDS Sample Buffer (2X) (Cat. No. LC1676) contain Dithiothreitol (DTT)?

Novex Tricine SDS Sample Buffer (2X), does not contain DTT or other reducing agents.

For Research Use Only. Not for use in diagnostic procedures.