Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer (2X)

Catalog No.	Quantity
LC2676	20 mL

Catalog No. LC2676 Supplier Invitrogen™ Supplier No. LC2676

Description

Use with Novex Tris-Glycine Gels

1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis

Order Info

Shipping Condition: Wet Ice

Specifications

• Chemical Name or Material: Sample Loading Buffer
• Concentration: 2X
• Physical Form: Liquid
• Product Line: Novex
• Quantity: 20 mL

Frequently Asked Questions (FAQs)

Where do I find buffer recipes for your precast protein gels?

The formulations of buffers for our precast protein gels can be found at this link: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-electrophoresis-buffers-reagents.html

What is the recipe for traditional Laemmli Buffer?

The Laemmli buffer or 2X SDS Buffer is composed of the following: 100 mM Tris HCl , pH 6.8, 200 mM dithiothreitol, 4% SDS, 0.2% bromophenol blue, 20% glycerol. 2X SDS gel loading buffer lacking dithiothreitol can be stored at room temperature. Dithiothreitol should then be added, just before use.

If a Tricine gel is accidentally run with buffers used in the Tris-Glycine system, what will happen and why?

If the Tricine gel is run with Tris-Glycine sample buffer, the bands will behave abnormally and resolve poorly. If the Tricine gel is accidentally run with Tris-Glycine running buffer, the gel will take longer to run and the resolution, especially for smaller proteins, will be worse than when the proteins are run on a Tris-Glycine gel with Tris-Glycine buffers. This is due to a combination of increase in stack area size (glycine is a slower ion than Tricine) and the higher ionic strength of the Tricine gel.

Why is LDS (lithium dodecyl sulfate) used in the 4X NuPAGE sample buffer instead of SDS?

SDS in a 4X sample buffer concentrate tends to precipitate from solution and to make the solution viscous and difficult to pipette. The LDS is much more soluble.

Can I use CTAB rather than SDS in my sample buffer?

No, CTAB will not work with any of our gels except for the NuPAGE Tris-Acetate gels. To use CTAB, you would need to use a running buffer of 50 mM acetic acid and 50 mM beta-alanine in equal concentrations. You would also need to switch the electrodes. Since CTAB is a cationic detergent, this would establish conditions for running a basic protein towards the anode (into the gel).

For Research Use Only. Not for use in diagnostic procedures.