Novus Biologicals™ pCL-10A1 Retrovirus Packaging Vector

Description

Applications: Retroviral Production

The pCL-10A1 packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a sell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of at the rate of about 1 infectious particle/cell/day Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. pCL-10A1 expresses 10A1 envelope protein that was isolated from a mouse infected with amphotropic MuLV (2,3). Retroviruses obtained by cotransfection with pCL-10A1 vector will infect most mammalian cells including hamster cells.

• Species: Human
• Specificity: Ectotropic (usually) (MoMuLV) Mouse and rat cells only (not human), Amphotropic (from 4070A MuLV) Most mammalian cells (but not hamster) 10A1 (MuLV) Most mammalian cells (including hamster)
• For large scale production, this plasmid may be amplified on LB plates containing 50μg/mL ampicillin
• Buffer: 10μg in 20μL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)

Recommended Storage

Store at -20°C. Avoid freeze-thaw cycles.

Specifications

• Storage Buffer: 10 μg in 1 x TE (10 mM Tris, pH 7.5, 1 mM EDTA)
• Content And Storage: Store at -20 C. Avoid freeze-thaw cycles.
• Species: Mouse, Mammal
• Concentration: 0.3 mg/mL
• Quantity: 10 μg
• Vector: pCL-10A1 Retrovirus Packaging Vector
• For Use With (Application): AMPLIFICATION: The Plasmid DNA Contains an Ampicillin Resistant Gene. For Large Scale Production, This Plasmid May be Amplified on LB Plates Containing 50 ug/ml Ampicillin. The Liquid Cultures May be Grown (at 100 ug Ampicillin/ml. Use in Retroviral Production Reported in Multiple Pieces of Scientific Literature.)