Novus Biologicals™ pCL-Eco Retrovirus Packaging Vector

Description

Applications: Retroviral Production

The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a sell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of about 1 infectious particle/cell/day. Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. Retroviruses obtained by cotransfection with pCL-Eco vector will infect mouse and rat cells, but not human cells.

• Species: Human, Mouse, Rat
• Specificity: Ectotropic (usually) (MoMuLV) Mouse and rat cells only (not human), Amphotropic (from 4070A MuLV) Most mammalian cells (but not hamster) 10A1 (MuLV) Most mammalian cells (including hamster)
• For large scale production, this plasmid may be amplified on LB plates containing 50μg/mL ampicillin
• Buffer: 10μg in 20μL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)

Specifications

• Storage Buffer: 10µg in 20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)
• Content And Storage: Store at -20°C. Avoid freeze-thaw cycles.
• Quantity: 10 μg
• Vector: pCL-Eco Retrovirus Packaging Vector