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Description
The pCMV-NS-OLLAS vector provides a convenient vector to express recombinant proteins under control of the CMV promoter with both an N-terminal OLLAS tag and a DYKDDDDK tag, protein will be secreted from the cell due to the inclusion of a signal peptide. Insert protein of interest into the vector in frame using the Xba I and EcoR I/Not I restriction sites. Bacterial selection can be performed using Kanamycin, while Neomycin resistance can be used for screening mammalian transfectants.
Recommended Storage
Store at -20°C. Avoid freeze-thaw cycles.
Specifications
Specifications
| Storage Buffer | 5mM Tris pH 8 |
| Content And Storage | Store at -20°C. Avoid freeze-thaw cycles. |
| Quantity | 0.01 mg |
| Vector | SD OLLAS/DYKDDDDK Vector |
| For Use With (Application) | For expression of secretory protein with N-terminally FLAG(DYKDDDDK) and OLLAS dual tandem tagged |
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