Invitrogen™ Human NrCAM ELISA Kit

Catalog No.	Quantity
EHNRCAM	96 Tests
EHNRCAMX10	10 x 96 Tests
EHNRCAMX5	5 x 96 Tests

Catalog No. EHNRCAM Supplier Invitrogen™ Supplier No. EHNRCAM

Description

ELISA

The Human NrCAM ELISA quantitates Hu NrCAM in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NrCAM. Principle of the method The Human NrCAM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Neuron-glia-related cell adhesion molecule (NRCAM) is a gene located on chromosome 7q31.1, encoding a protein that belongs to the immunoglobulin superfamily of cell adhesion molecules. NRCAM is expressed in the nervous system and plays a vital role in the development and maintenance of neural connections. This adhesion molecule facilitates neuron-neuron and neuron-glia interactions, impacting processes such as axon guidance, synapse formation, and cell signaling, which are crucial for neural development and plasticity. NRCAM is involved in the modulation of signal transduction pathways that influence neuronal growth and repair, contributing to the stabilization of synaptic structures. Alterations in NRCAM expression or function have been linked to neurodevelopmental disorders, including autism spectrum disorders, and certain psychiatric conditions, reflecting its importance in maintaining neural circuit integrity. Research into NRCAM continues to explore its therapeutic potential in neurobiology and its implications in neurological and psychiatric disorders.

Specifications

• Accession Number: Q92823
• Assay Range: 0.328-80 ng/mL
• Assay Sensitivity: 0.3 ng/mL
• Calibration Range: 0.3 ng/mL to 80 ng/mL
• Content And Storage: Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA pair antibody detects human NrCAM. Other species not determined.
• Sufficient For: 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: ankyrin-binding cell adhesion molecule NrCAM; Bravo; C030017F07Rik; C130076O07Rik; hBravo; KIAA0343; mBravo; MGC138845; MGC138846; mKIAA0343; neuronal cell adhesion molecule; Neuronal surface protein Bravo; neuron-glia-CAM-related cell adhesion molecule; ng-CAM-related; NgCAM-related cell adhesion molecule; Nr CAM; Nrcam; nr-CAM; rBravoankyrin-binding cell adhesion molecule NrCAM; Bravo; C030017F07Rik; C130076O07Rik; hBravo; KIAA0343; mBravo; MGC138845; MGC138846; mKIAA0343; neuronal cell adhesion molecule; Neuronal surface protein Bravo; neuron-glia-CAM-related cell adhesion molecule; ng-CAM-related; NgCAM-related cell adhesion molecule; Nr CAM; Nrcam; nr-CAM; rBravo;
• Gene ID (Entrez): 4897
• Gene Symbol: NRCAM
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Angiogenesis
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: N/A
• Target: NRCAM
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min