Thermo Scientific™ Nuclear and Cytoplasmic Protein Extraction Bundle

Description

Includes

NE-PER Nuclear and Cytoplasmic Extraction Reagents (15 mL), Halt Protease Inhibitor Cocktail (100X) (1 mL), Micro BCA Protein Assay Kit (500 mL)

Nuclear and Cytoplasmic Protein Extraction Bundle provides a simple method for extracting nuclear and cytoplasmic proteins and high-precision total protein concentration determination. The bundle includes Thermo Scientific NE-PER Kit, Halt Protease Inhibitor Cocktail and Micro BCA Protein Assay Kit

• Thermo Scientific NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilize and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA
• Halt Protease Inhibitor cocktail protects proteins from proteolytic degradation through inhibition of serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples. Once desalted or diluted, the isolated proteins can be used to perform immunoassays and protein interaction experiments, such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays
• The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. The assay is exceptionally linear and exhibits very low levels of protein-to-protein variability and accurately measures 0.5 to 20µg/mL protein solutions

Specifications

• Quantity: 1 Bundle
• Includes: NE-PER Nuclear and Cytoplasmic Extraction Reagents (15 mL), Halt Protease Inhibitor Cocktail (100X) (1 mL), Micro BCA Protein Assay Kit (500 mL)
• Content And Storage: See instructions for individual components
• Column Type: Spin Column