The Cell Line Nucleofector Kit V is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b System. This kit has been determined to be optimal for transfecting following cell types:32D3T3-L1pre-adA20A375A7r5AGSARPE-19B16-F10BA/F3 (DSMZ)C2C12C6Capan-1CHO [suspension]CHO-S [suspension] (Life Technologies)COS-1HaCaTHCT116HEK293HepG2HL-60HUV-EC-CJurkatK-562 (DSMZ)MCF7MDA-MB-231MDA-MB-468NB-4 (DSMZ)NCTC clone 929Neuro-2a [N2a]NG108-15P19PC-12PC-3RajiRamosRAW 264.7RBL-1S49Saos-2Schneider‘s Drosophila Line 2SH-SY5YSK-N-SHSK-OV-3SW480T/C-28 a2T/GHA-VSMCT-47DTHP-1U-2 OSVeroBenefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 × 10⁵ to 2 × 10⁷ cells Low-throughput transfection in single cuvette format