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Biotium NucView 405 Caspase-3 Substrate, 1 mM in DMSO
Supplier: Biotium 10405
NucView Caspase-3 Substrates are novel fluorogenic substrates for real-time or endpoint detection of apoptosis in live cells. The non-toxic, no-wash substrates do not inhibit caspase-3 unlike other caspase substrates or FLICA assays permitting multiday analysis. They consist of a high-affinity DNA dye linked to a DEVD caspase-3 substrate moiety. Initially non-fluorescent, the cell permeable substrate is cleaved by caspase-3 in apoptotic cells and emits fluorescence, for detection of caspase-3 activity and apopototic nuclear morphology. Suitable for flow cytometry, fluorescence microscopy, or live cell imaging systems, staining is compatible with fixation and permeabilization for immunostaining. NucView 488 is validated in over 100 cell types and 200 publications. Available with blue (NucView 405), green (NucView 488), or red (NucView 530) fluorescence, as PBS solutions for DMSO-sensitive cells, and as stand-alone assays or in kits with other apoptosis or necrosis probes.
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