Thermo Scientific™ Nunc™ Biobanking and Cell Culture Cryogenic Tubes

Catalog No.	Bottom Shape	Capacity (Metric)	Thread Style	Style
12-565-161N	Conical (Self-standing)	4.5 mL	Internal	Writing Surface, A-R Base
12-570-216	Conical (Self-standing)	0.5 mL	Internal	No Writing Surface, A-R Base
12-570-217	Conical (Self-standing)	1 mL	Internal	Writing Surface
12-565-164N	Conical (Self-standing)	1 mL	External	Writing Surface, A-R Base
12-565-297	Conical (Self-standing)	1 mL	External	No Writing Surface, A-R Base
12-565-168N	Conical (Self-standing)	1 mL	Internal	Writing Surface, A-R Base
12-565-169N	Conical (Self-standing)	1 mL	Internal	Writing Surface
12-565-163N	Round (Self-standing)	1.8 mL	External	Writing Surface, A-R Base
12-565-298	Round (Self-standing)	1.8 mL	External	No Writing Surface, A-R Base
12-565-167N	Round (Self-standing)	1.8 mL	Internal	Writing Surface, A-R Base
12-565-171N	Round (Self-standing)	1.8 mL	Internal	Writing Surface
12-565-170N	Round	1.8 mL	Internal	Writing Surface
12-565-162N	Round (Self-standing)	3.6 mL	Internal	Writing Surface, A-R Base
12-565-172N	Round	3.6 mL	Internal	Writing Surface
12-566-98	Round	3.6 mL	Internal	Writing Surface, No Cap
12-565-291	Round (Self-standing)	4.5 mL	External	Writing Surface, A-R Base
12-565-299	Round (Self-standing)	4.5 mL	External	No Writing Surface, A-R Base
12-565-173N	Round	4.5 mL	Internal	Writing Surface

Catalog No. 12-565-161N Supplier Thermo Scientific™ Supplier No. 379146

Description

Store samples from general cold storage to the vapor phase of liquid nitrogen with these biobanking and cell culture cryogenic tubes, available with either internal or external threads.

Store samples from general cold storage to the vapor phase of liquid nitrogen with Thermo Scientific™ Nunc™ Biobanking and Cell Culture Cryogenic Tubes, available with either internal or external threads.

• Non-cytotoxic, non-mutatgenic raw materials and pyrogen-free IVD compatible tubes for added sample security
• External or internally threaded options available
• Certified SAL of 10^-6 for the highest sterility assurance available
• Suitable for storage from general cold storage refrigeration (+4°C) down to the vapor phase of liquid nitrogen
• Bulk, uncapped, non-sterile versions available for less demanding applications

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Specifications

• Product Type: Cryogenic Storage Tube
• Diameter (Metric): 12.5 mm
• Closure Color: Clear
• Diameter (English) Outer: 0.492 in.
• Capacity (Metric): 4.5 mL
• Bottom Shape: Conical (Self-standing)
• Material: Polypropylene
• Thread Style: Internal
• Length (English): 3.622 in.
• Length (Metric): 92 mm
• Packaging: 300
• Style: Writing Surface, A-R Base
• RNase Free: Yes
• Vapor LN2 (to -150°C): Yes
• Product Line: Nunc
• Sterility: Sterile
• Anti-Rotation: Yes
• Packaging Quantity: 300 Pk.
• Closure Material: Polypropylene
• Capacity (English): 0.152 oz.
• Dnase Free: Yes
• Closure Type: Screw Cap
• Printed: Yes

Frequently Asked Questions (FAQs)

Will the heat required to properly seal Nunc CryoFlex Tube Wrap adversely affect my cells?

Nunc Biobanking and Cell Culture Cryogenic Tubes are normally placed in a 4 degrees C ice bath during cell inoculation in the cryogenic tube. The heat required to shrink the polyethylene Nunc CryoFlex Tube Wrap will not raise the internal temperature enough to damage the cells. At this stage, cryoprotective agents are added.

What protocol should I follow for thawing cells, if my Nunc Biobanking and Cell Culture Cryogenic Tubes were previously immersed in liquid nitrogen without Nunc CryoFlex Tube Wrap?

We recommend the following protocol for thawing cells immersed in liquid nitrogen without Nunc CryoFlex Tube Wrap:
- Wear appropriate protective clothing including gloves, face guard, etc., during all transfer processes.
- The Nunc Biobanking and Cell Culture Cryogenic Tubes should be stored at least 24 hr in the gas phase of the liquid nitrogen freezer. This should allow trapped liquid nitrogen to evaporate and escape slowly, thereby reducing the possible pressure in the cryogenic tube.
- After at least 24 hr storage in the gas phase, the cryogenic tube may be put into a water bath and covered with a towel.
- After thawing, the contents of the cryogenic tube can be transferred.

Can I immerse Nunc Biobanking and Cell Culture Cryogenic Tubes in liquid nitrogen (liquid phase) for storage?

Of the Nunc Biobanking and Cell Culture Cryogenic Tubes, we recommend the internally threaded design, incorporating a silicon gasket, for liquid nitrogen immersion. At ultra low temperatures, any sealing material becomes inflexible. A theoretical risk of leakage is present when any mechanical closure is used. To facilitate leakproof storage, mechanically closed containers must be hermetically sealed in a protective envelope.

The Nunc cryo products includes Nunc CryoFlex Tube Wrap, a heat sealable polyethylene tubing designed for cryogenic tube encasement. The use of cryogenic tubes for freezing in liquid nitrogen (liquid phase) storage without CryoFlex tubing poses a safety hazard.
WARNING: Do not use Nunc Biobanking and Cell Culture Cryogenic Tubes in liquid phase of liquid nitrogen unless correctly sealed in Nunc CryoFlex Tube Wrap (Cat. No. 343958). Improper use may cause entrapment of liquefied nitrogen inside the vial and lead to pressure build-up, resulting in possible explosion or biohazard release. Use appropriate safety procedures as outlined in the Nalge Nunc International Cryopreservation Manual when handling and disposing of the vials.

What is the difference between your Nunc Biobanking and Cell Culture Cryogenic Tubes with internal threads and external threads?

Internally threaded Nunc Biobanking and Cell Culture Tubes are designed for vapor phase liquid nitrogen freezing. The cap incorporates a silicon gasket to enhance sealing properties.
Externally threaded Nunc Biobanking and Cell Culture Tubes are designed for mechanical freezing. The external screw cap reduces risk of contamination due to handling.

Can I autoclave Nunc Biobanking and Cell Culture Cryogenic Tubes?

We do not recommend autoclaving Nunc Biobanking and Cell Culture Tubes, even though they are made of polypropylene with polyethylene caps and stoppers.

Should I choose externally or internally threaded cryostorage vials?

Both types are designed and tested for secure cryogenic storage, so it’s largely a matter of personal preference. In an externally threaded vial (in which the cap covers the entire top portion of the vial itself), the underside of the cap is protected when unscrewed), which can minimize the potential for cross-contamination. In an internally threaded vial (in which the vial threads are inside the neck area and the cap inserts like a plug), the cap is the same diameter as the vial itself which can be helpful in certain types of dense storage applications.

Can I use your Nunc cryotubes or Nalgene cryotubes in liquid nitrogen?

Liquid nitrogen has very low surface tension, so it can flow into any of our cryostorage vials even if they are tightly capped. This creates significant potential for cross-contamination in storage as well as risk of injury from explosion when the vials are removed from storage and the liquid nitrogen boils off. The only option to mitigate this is Nunc Cryoflex tubing (e.g., Cat. No. 343958), which results in a heat-sealed barrier layer around each vial. Alternatively, storage in the vapor phase of liquid nitrogen is acceptable unless otherwise noted.