Thermo Scientific™ Nunc™ Lab-Tek™ II Chamber Slide™ System

Catalog No.	Wells
12-565-6	2
12-565-5	1
12-565-7	4
12-565-8	8

Catalog No. 12-565-6 Supplier Thermo Scientific™ Supplier No. 154461

Description

Grow cells directly on slides with the Nunc Lab-Tek II Chamber Slide System.

Grow, stain, and examine cells directly on the Thermo Scientific Nunc Lab-Tek II Chamber Slide, which consists of a removable polystyrene media chamber attached to a standard glass slide. The slide surface is specially treated for adherent cell culture. Available in 1-, 2-, 4-, and 8-well formats.

Features

• Slide surface that is specially treated with a unique RS wash to ensure consistent cell growth
• Tabbed polystyrene lid that allows gas exchange with minimal evaporation
• Removal tool that separates media chamber from glass slide
• Biocompatible adhesive hydrophobic well border printed on slide
• White frosted writing area

Includes: chamber removal tool

Additional information

• Can be purchased in case of 96 or pack of 16
• Shipped sterile in convenient stackable incubation trays

Warranty: 90 days

Specifications

• Wells: 2
• Culture Area: 4.0 cm²
• Lid Material: Polystyrene
• Description: 2-well Chamber Slide w/ removable wells
• Surface Treatment: Cell Culture
• Material: RS Glass
• Volume (Metric) Working: 1.0 to 2.0 mL
• Includes: Clear polystyrene lid with handles on the top and side for aseptic manipulations
• No. per Pack: 16
• Dimensions (LxW): 74 x 25 mm
• Sterility: Sterile
• Closure Color: Clear
• Quantity: 1 Ea.

Frequently Asked Questions (FAQs)

What cell density should I use for Lab-Tek Chambered Slides and Lab-Tek II Chambered Slides?

Unfortunately, we do not have a recommended cell density for our LabTek Chamber Slides. However, you can refer to the following link, which provides recommended cell densities for our other cell culture products:
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/cell-culture-useful-numbers.html

Are your Nunc Lab-Tek II Chamber Slide System products pre-coated?

The Nunc Lab-Tek II Chamber Slide System products are not coated with any chemicals or reagents.

What is the difference between your original Nunc Lab-Tek Chamber Slide System and your Nunc Lab-Tek II Chamber Slide System?

Please review the following key differences between the Nunc Lab-Tek Chamber Slide System and the Nunc Lab-Tek II Chamber Slide System:
- The Nunc Lab-Tek Chamber Slide System is available in Permanox plastic or glass and incorporates a silicone gasket.
- The Nunc Lab-Tek II Chamber Slide System is available in glass only and do not have a silicone gasket.
- The Nunc Lab-Tek II Chamber Slide System has a blue hydrophobic border and the upper structure is attached by an adhesive. Supplied with this product is a key and holder system which is used to remove the upper structure from the glass slide.
- The Nunc Lab-Tek Chamber Slide System is available in 1-, 2-, 4-, 8- or 16-well formats.
- The Nunc Lab-Tek II Chamber Slide System is available in 1-, 2-, 4- or 8-well formats.

On the packaging of your Nunc Lab-Tek II Chamber Slide System, it states these chamber slides have an RS-treated growth surface. What is RS treatment?

The RS treatment is a wash step formulated to improve the consistency of the glass surface of the Nunc Lab-Tek II Chamber Slide System. RS glass treatment prepares the glass surface for culture after the hydrophobic border has been applied.
The term "RS treatment" refers to the glass washing procedure.

What stains and fixatives are compatible with Lab-Tek chambered slides and coverglasses?

Lab-Tek products are compatible with all common fixatives, including paraformaldehyde. Acetone at 100% and some mixtures of acetone and alcohols are not compatible with the polystyrene upper well structure and will result in clouding or other effects of chemical attack. We encourage careful testing within planned operating conditions before working with high-value samples.

What factors must I consider when working with Lab-Tek and Lab-Tek II chambered coverglass products?

Lab-Tek chambered coverglasses are not accessories to Lab-Tek chamber slides, but another complete product for cell culture. The chambers are simply adhered to a borosilicate coverglass (to serve as the culture surface) rather than a soda lime glass or Permanox microscope slide. Most customers select this format for compatibility with confocal imaging systems that require a #1.0, approx. 120 micron (Lab-Tek) or #1.5, approx. 180 micron (Lab-Tek II) substrate. Because the coverglass is very thin and fragile, the chambered coverglasses (unlike chamber slides) cannot be disassembled.

What are the different options available in Lab-Tek II chamber slides?

Lab-Tek II chambered glass slide systems are available in our standard high-purity water washed culture treatment or our “CC2” surface modification. The latter is a proprietary passive treatment that resembles poly-D-lysine, often improving culture performance when working with fastidious cells such as neuronal cultures.

What are the different options available in Lab-Tek chamber slides?

Lab-Tek chambered slide systems are based either on a standard soda lime glass microscope slide, or a culture-treated Permanox plastic slide. The latter may serve as a better culture surface for certain types of cells that don't grow well on glass, but may be of limited use for certain types of fluorescence microscopy due to the endogenous autofluorescence of plastic.

What is the cell culture treatment performed on glass Lab-Tek slides?

The standard glass Lab-Tek slide cell culture treatment consists of a proprietary, multi-stage high-purity water washing process. Unlike our plastic products for adherent cell culture (see Nunclon Delta), there is no need for further modification of the surface because glass is already hydrophilic and therefore already suitable for culture of many adherent cell lines.

What are the differences between Lab-Tek and Lab-Tek II chamber slide systems?

The main difference is the way the upper chamber structure is attached to the slide. Lab-Tek slides use a silicone gasket that can be peeled away once the chambers are removed after culture. Lab-Tek II slides use a hydrophobic acrylic adhesive that remains on the slide after chamber removal but (due to its very low profile) will not interfere with coverslips. The chamber geometry is also slightly different: on Lab-Tek slides the walls lean inward slightly to limit media evaporation, whereas on Lab-Tek II slides they are completely vertical.

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