U-Blot Pan-O-GlcNAc Rabbit mAb (Recombinant Monoclonal Clone W-R7) is a Rabbit-derived recombinant monoclonal antibody (clone W-R7) that specifically recognizes O-GlcNAc a o-glcnacylation modification at Ser(S)/Thr(T) It shows reactivity with Human Mouse Rat Validated applications include WB IHC IF ELISA Recommended dilutions WB 1200-11000 IF 1200-11000 ELISA 15000-120000 Purified by recombinant antibody expressed in animal component-free (acf) media purified via protein a affinity chromatography O-GlcNAc (short for O-linked -N-acetylglucosamine) is a reversible enzymatic post-translational modification found on serine and threonine residues of nucleocytoplasmic proteins The modification is characterized by a -glycosidic bond between the hydroxyl group of serine or threonine side chains and N-acetylglucosamine (GlcNAc) Supplied as 100 uL