MP Biomedicals™ O-Nitrophenyl-β-D-Galactopyranoside, MP Biomedicals™

Useful in the detection of lacZ activity. O-Nitrophenyl-β-D-Galactopyranoside, MP Biomedicals is a colorimetric substrate for b-galactosidase and a cell culture component for molecular genetics. ONPG reactions are rapid and occur within a 24-hour per

Supplier:  MP Biomedicals™ 02102473.5

Catalog No. MP021024735

Description

This product is hydrolyzed to O-Nitrophenol by the enzyme. O-Nitrophenol is yellow in alkaline solution and can be quantitated at 410-420nm².

• β-galactosidase breaks down lactose into galactose and glucose
• β-galactosidase is not lactose specific and can act on simple galactosides
• 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound
• The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell

Specifications

• Form: Solid
• Quantity: 500mg
• Molecular Weight (g/mol): 301.3
• Storage Requirements: 4°C

• For Use With (Application): o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.
• Format: White Solid
• pH: −66° ± 7° (c=1, H2O)