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MP Biomedicals™ O-Nitrophenyl-β-D-Galactopyranoside, MP Biomedicals™

Catalog No. MP021024735 Shop All MP Biomedicals, Inc Products
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MP021024735 500mg
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Catalog No. MP021024735 Supplier MP Biomedicals™ Supplier No. 02102473.5
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Description

Useful in the detection of lacZ activity. O-Nitrophenyl-β-D-Galactopyranoside, MP Biomedicals is a colorimetric substrate for b-galactosidase and a cell culture component for molecular genetics. ONPG reactions are rapid and occur within a 24-hour per

This product is hydrolyzed to O-Nitrophenol by the enzyme. O-Nitrophenol is yellow in alkaline solution and can be quantitated at 410-420nm2.

  • β-galactosidase breaks down lactose into galactose and glucose
  • β-galactosidase is not lactose specific and can act on simple galactosides
  • 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound
  • The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell
TRUSTED_SUSTAINABILITY

Specifications

Form Solid
For Use With (Application) o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.
Quantity 500mg
Format White Solid
Molecular Weight (g/mol) 301.3
pH −66° ± 7° (c=1, H2O)
Storage Requirements 4°C
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