The Cell Line Optimization 4D-Nucleofector X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector X Unit.Benefits:Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experimentOptimal Nucleofection conditions determined in the 16-well Nucleocuvette Strips are transferable to the 100 μL single Nucleocuvette in the 4D-Nucleofector X UnitConditions are transferable between 4D Nucleofector System, 96-well Shuttle System and 384-well Nucleofector SystemApplications:Determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector X UnitContent:0.675 mL Nucleofector Solution SE0.675 mL Nucleofector Solution SF0.675 mL Nucleofector Solution SG3 x 0.15 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0) 4 x 20 µL 16-well Nucleocuvette Strips