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Enzo Life Sciences SEEBRIGHT Orange 552 dUTP (25nmol)
SDP

Catalog No. 502012548
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SEEBRIGHT Orange 552  dUTP (5-TAMRA-dUTP) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. SEEBRIGHT Orange 552 dUTP can also be used for multicolor fluorescence labeling. Alternative name: 5-TAMRA dUTP. Concentration: 1mM. Quantity: Sufficient for approximately 49 reactions, following the recommended protocol of ENZ-GEN111. Purity: ≥93% (HPLC). Formulation: Liquid. Solution in water.

Catalog No. 50-201-2548 Supplier Enzo Life Sciences Supplier No. ENZ42842
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SEEBRIGHT Orange 552  dUTP (5-TAMRA-dUTP) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. SEEBRIGHT Orange 552 dUTP can also be used for multicolor fluorescence labeling. Alternative name: 5-TAMRA dUTP. Concentration: 1mM. Quantity: Sufficient for approximately 49 reactions, following the recommended protocol of ENZ-GEN111. Purity: ≥93% (HPLC). Formulation: Liquid. Solution in water.

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