Invitrogen™ Human Osteoprotegerin (TNFRSF11B) Instant ELISA™ Kit

Catalog No.	Quantity
50-178-98	128 Tests

Catalog No. 50-178-98 Supplier Invitrogen™ Supplier No. BMS2021INST

Description

ELISA

The Human Osteoprotegerin (TNFRSF11B) (Hu OPG) ELISA quantitates Hu OPG in human serum, plasma, cell culture supernatants or other body fluids. The assay will exclusively recognize both natural and recombinant Hu OPG. Principle of the method The Human OPG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.

Specifications

• Accession Number: O00300
• Assay Range: 15.6-1000 pg/mL
• Assay Sensitivity: 2.5 pg/mL
• Content And Storage: Aluminum pouch(es) with a Microplate coated with Polyclonal Antibody to OPG, Biotin-Conjugate (anti-OPG polyclonal antibody), Streptavidin-HRP and Sample Diluent, lyophilized
  Aluminum pouch(es) with a human Osteoprotegerin Standard curve (coloured)
  Wash Buffer Concentrate 20x (phosphate-buffered saline with 1% Tween 20)
  Substrate Solution (tetramethyl-benzidine)
  Sample Diluent (Use when an external predilution of the samples is needed)
  Stop Solution (1M Phosphoric acid)
  Adhesive Plate Covers
• Conjugate: HRP
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: LOW QUALITY PROTEIN: tumor necrosis factor receptor superfamily member 11B; MGC29565; OCIF; Opg; osteoclastogenesis inhibitory factor; Osteoprotegerin; PDB5; soluble OPG; soluble Osteoprotegerin; sOPG; TNF receptor superfamily member 11b; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily member 11B; tumor necrosis factor receptor superfamily, member 11b; tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)LOW QUALITY PROTEIN: tumor necrosis factor receptor superfamily member 11B; MGC29565; OCIF; Opg; osteoclastogenesis inhibitory factor; Osteoprotegerin; PDB5; soluble OPG; soluble Osteoprotegerin; sOPG; TNF receptor superfamily member 11b; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily member 11B; tumor necrosis factor receptor superfamily, member 11b; tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin); OCIF; OPG; PDB5; TR1
• Gene ID (Entrez): 4982
• Gene Symbol: TNFRSF11B
• Interassay CV: 8%
• Intraassay CV: 7%
• Kit Contents: Pre-coated 96 well Microplate, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers, Biotinylated Detection Antibody, SAV-HRP, Sample Diluent, lyophilized, Standard (8-well strips x 4), Wash Buffer Concentrate, Substrate Solution, Sample Diluent, Stop Solution, Lysis Buffer 10x, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Osteoclastogenesis inhibitory factor, Osteoprotegerin
• Quantity: 128 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
• Storage Requirements: -20°C
• Synonym: Osteoprotegerin, TNF-R superfamily member 11 b
• Target: TNFRSF11B
• Test Time: 15 min
• Total Assay Time: 3 hr 10 min