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Thermo Scientific™ Biocemical Identification System (O.B.I.S.) PYR
Description
Rapidly determine PYRase activity is streptococci and Citrobacter spp. with this biochemical identification system.
Rapidly determine PYRase activity is streptococci and Citrobacter spp. with Thermo Scientific™ Oxoid™ Biochemical Identification System (O.B.I.S.) PYR. This rapid colorimetric test delivers simple and rapid results for the determination of PYRase activity in streptococci and Citrobacter spp, offering a rapid diagnostic alternative to time-consuming culture methods. In addition, the same enzymatic activity may be used to aid differentiation of Salmonella spp. from Citrobacter spp and other Enterobacteriaceae1,2.
Use O.B.I.S. PYR as a rapid diagnostic alternative to time-consuming culture methods.
- Simple—easy to implement
- Rapid—results in 6 minutes of testing suspect colonies
- Non-carcinogenic substrates
The O.B.I.S. - PYR test uses Test Cards impregnated with L-pyroglutamic acid 7-amino-4-methyl-coumarin (7AMC) and dimethylaminocinnamaldehyde for the detection of PYRase activity. The enzymatic hydrolysis of this substrate by enterococci, Group A streptococci and Citrobacter spp. produces a purple color following the addition of the Developing Solution3.
We have developed a new system for PYRase testing4 using a non-carcinogenic substrate in response to health concerns associated with the use of β-naphthylamide (a potent carcinogen)5. The PYRase area on the O.B.I.S. PYR test is impregnated with L-pyroglutamic acid 7-amino-4-methylcoumarin (7AMC)4. Dimethylaminocinnamaldehyde is used as a color development reagent. The enzymatic hydrolysis of the substrate produces a purple color on addition of the O.B.I.S. PYR Developing Solution4.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.

Specifications
Specifications
Content And Storage | 2°C to 8°C |
Description | O.B.I.S. PYR |
Detectable Analytes | PYRase activity |
Includes | Test cards, developing solution, buffer solution |
Quantity | 60 Tests/Kit |
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