Invitrogen™ Oxytocin Competitive ELISA Kit

Catalog No.	Quantity
EEL139	96 Tests

Catalog No. EEL139 Supplier Invitrogen™ Supplier No. EEL139

Description

ELISA

The Oxytocin (OT) ELISA quantitates OT in serum, plasma and other biological fluids. Principle of the method The Competitive Oxytocin (OT) ELISA research use only kit is designed to quantitatively measure OT independent of species. A OT standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to OT is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of oxytocin in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

There are two proteins encoded by this gene, oxytocin and neurophysin I. Oxytocin is posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. The precursor seems to be activated while it is being transported along the axon to the posterior pituitary. This hormone contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.

Specifications

• Assay Range: 15.63-1000 pg/mL
• Assay Sensitivity: 9.38 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: All Species
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: neurophysin 1; neurophysin I; Ocytocin; Ot; OT-NPI; oxcytocin; OXT; OXT-NPI; Oxy; Oxytocin; oxytocin, prepro- (neurophysin I); oxytocin, prepropeptide; oxytocin/neurophysin (Oxt) gene, complete gene, complete cds; oxytocin/neurophysin 1 prepropeptide; oxytocin/neurophysin I prepropeptide; oxytocin-neurophysin 1; oxytocin-neurophysin I; oxytocin-neurophysin I, preproprotein; prepropeptide for oxytocin and neurophysin; unnamed protein productneurophysin 1; neurophysin I; Ocytocin; Ot; OT-NPI; oxcytocin; OXT; OXT-NPI; Oxy; Oxytocin; oxytocin, prepro- (neurophysin I); oxytocin, prepropeptide; oxytocin/neurophysin (Oxt) gene, complete gene, complete cds; oxytocin/neurophysin 1 prepropeptide; oxytocin/neurophysin I prepropeptide; oxytocin-neurophysin 1; oxytocin-neurophysin I; oxytocin-neurophysin I, preproprotein; prepropeptide for oxytocin and neurophysin; unnamed protein product;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Tissue Homogenate 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Oxytocin
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min