Gibco™ N-2 Supplement (100X)

Catalog No.	Quantity
17-502-048	5 mL
17-502-001	50 mL

Catalog No. 17-502-048 Supplier Gibco™ Supplier No. 17502048

Description

N-2 Supplement is a versatile serum-free cell culture supplement that supports the growth and viability of various cell types.

Gibco N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein's N-1 formulation, containing optimized concentrations of insulin, iron-rich holo-transferrin, progesterone, putrescine, and selenite. N-2 Supplement is recommended for growth and expression of neuroblastomas and neural cells in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS).

N-2 Supplement has been demonstrated in wide-ranging applications, such as the culture of neural stem cells, the culture of the SH-SY5Y cell line (subline of the neuroblastoma cell line SK-N-SH), and culture of the HT22 mouse hippocampal neuronal cell line. Additionally, N-2 Supplement adds support to cell culture media used to differentiate pluripotent stem cells (PSCs) to a variety of cell types, including neural and pancreatic-like cell types.

N-2 Supplement may be used with Neurobasal media supplemented with growth factors such as bFGF and EGF. When combined with Dulbecco's Modified Eagle Medium (D-MEM) and fetal bovine serum (FBS), N-2 Supplement also provides suitable supplementation for the culture of human and rodent astrocytes.

• Each lot is cGMP manufactured
• Each lot is tested for sterility, endotoxin, and performance
• Contains insulin, iron-rich holo-transferrin, progesterone, putrescine, and selenite
• Can be used as a substitute for Bottenstein's N-1 formulation
• Provided as a 100X concentrate

Choose Gibco N-2 Supplement for a reliable, high-quality product trusted by researchers worldwide.

Specifications

• Content And Storage: Store in freezer (-5 to -20°C) and protect from light.
  
  
• Cell Type: Neural Cells
• Classification: Serum-free
• Concentration: 100 X
• Culture Type: Mammalian Cell Culture
• Form: Liquid
• Product Type: Cell Culture Supplement
• Serum Level: Serum-Free
• Sterility: Sterile
• pH: 6 to 8
• Quantity: 5 mL
• Shipping Condition: Dry Ice
• Tested For: Endotoxin, Performance, Sterility

Frequently Asked Questions (FAQs)

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Is there a difference between the Gibco N-2 Supplement formulation from Thermo Fisher and the N-2 Supplement formulation from Global Stem?

Both N-2 Supplement formulations can be used in the culture of neurons, but the concentrations of components in the formula for the Global Stem N-2 Supplement are higher than the Gibco N-2 Supplement.

Can I filter the N-2 Supplement?

The N-2 Supplement (100X) solution can be filtered. We do not recommend filtering the medium once N-2 has been added at a final concentration of 1X.

What is the recommended storage condition and shelf life for N-2 Supplement (100X) (Cat. No. 17502001 or 17502048)?

The shelf life for N-2 Supplement (100X) is 18 months from the date of manufacture when stored at the recommended storage condition of -5 to -20 degrees C, protected from light. The expiration date should be listed on the product label as well as on the COA for the lot of the supplement.

Which supplement can I add to Neurobasal Medium to culture my neural cells?

As a general guideline, we recommend using Gibco B-27 Plus Supplement to culture neural stem cells, hippocampal, and other CNS neurons.

- For neuroblastomas, or post-mitotic neurons from both PNS and CNS, Gibco N-2 Supplement can be used.
- For primary glial cells (astrocytes) or tumor cell lines of astrocytic phenotype (astrocytes and gliomas), or oligodendrocytes, Gibco G-5 Supplement can be used.

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.