Gibco™ L-Glutamine (200 mM)

Catalog No.	Quantity
25-030-081	100 mL
25-030-149	20 mL
25-030-164	20 x 100 mL

Catalog No. 25-030-081 Supplier Gibco™ Supplier No. 25030081

Description

Gibco L-Glutamine is a ready-to-use amino acid supplement for cell culture. L-glutamine is an amino acid required in cell culture for a variety of critical cellular processes.

Gibco L-Glutamine is a ready-to-use amino acid supplement for cell culture. L-glutamine is an amino acid required in cell culture for the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. Unlike most other amino acids, L-glutamine is not stable in solution with a rate of degradation dependent upon time, temperature, and pH. The optimal concentration depends upon the cell type and medium used to culture the cells, but generally falls in the range of 2–6 mM.

Gibco L-Glutamine supplement is a ready-to-use 200 mM stock solution for cell culture supplementation that supports of many critical cellular functions, including:

• Formation of purine and pyrimidine nucleotides
• Formation of amino sugars, glutathione, and L-glutamate
• Formation of other amino acids
• Protein synthesis
• Glucose production

cGMP manufacturing and quality system

For supply chain continuity, we manufacture this product at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.

Order Info

Shipping Condition: Dry Ice

Specifications

• Chemical Name or Material: Glutamine
• pH: 5 to 6
• Quantity: 100 mL
• Concentration or Composition (by Analyte or Components): 100X
• Physical Form: Liquid
• Product Line: Gibco
• Shipping Condition: Dry Ice
• Green Features: Sustainable packaging

Frequently Asked Questions (FAQs)

Why did my 200 mM L-Glutamine precipitate out of solution when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Why does L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution have “Product of Brazil” on the label?

L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution is made in the USA from a raw material glutamine powder manufactured in Brazil. The country of manufacture of a component that has been subjected to manufacturing or processing operations in more than one country is the last country in which the component has been “substantially transformed”. A “substantial transformation” occurs in a country when a product emerges from a manufacturing process with a name, character, or use different from that of the original material(s) subjected to that process.

Diluting, purifying, sorting, testing, packing, repacking, or affixing labels to a product in a country does not constitute a “substantial transformation” in that country, hence the label must bear the country of the original raw material origin.

My order of L-Glutamine (Cat. No. 25030081) arrived frozen. Is it stable?

If L-Glutamine is completely or partially frozen, it is still stable. It becomes unstable when stored for extended periods of time completely thawed, especially above 2-8 degrees C.

What is your recommended method for thawing L-Glutamine before adding to cell culture media?

We recommend thawing at 2-8 degrees C and then warming at 37 degrees C until the material goes into solution. Mix it as it warms up to spend minimal time at elevated temperatures before you aliquot it.

Why did my 200 mM L-glutamine precipitate when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37 degrees C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.