The P3 Primary Cell 4D-Nucleofector LV Kit L is suited for larger scale transfection of primary cells in the 1 mL Nucleocuvette Cartridge format when working with the 4D-Nucleofector LV Unit. With the 1 mL Nucleocuvette Cartridge a fixed volume of 1 mL can be transfected. The cartridge is manually filled via a sterile injection port.Applications:For large scale transfection of up to 1 x 10⁸ cellsEx-vivo modification of human primary cells for the development and establishment of cell therapy applications (e.g. genome editing generation of CAR-T cells)Generation of large numbers of transiently modified primary cells for cell-based assaysContent:2.25 mL P3 Primary Cell Nucleofector Solution0.5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)2 x 1 mL Nucleocuvette Cartridge