Thermo Scientific™ PageRuler™ Unstained High Range Protein Ladder

Description

A mixture of 8 unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

The Thermo Scientific™ PageRuler Unstained High Range Protein Ladder is a mixture of 8 unstained proteins (60 to 250kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and Western blotting.

The protein MW markers in this ladder resolve into clearly identifiable, sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 150kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained High Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Highlights:

• Size range 8 proteins spanning 60 to 250kDa
• Ready-to-use supplied in a loading buffer for direct loading on gels
• Sharp bands excellent accuracy for detection with coomassie or silver stains
• Reference band 150kDa band has greater intensity for easy orientation
• Tagged each protein in the ladder contains an integral Strep-tag™ II Sequence

• Quality tested each lot evaluated by SDS-PAGE and Western blotting

Includes:

• Proteins (0.02 to 0.05mg/mL of each) in 62.5mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN₃, 0.01% bromophenol blue and 33% glycerol

Recommended for:

• Accurate protein sizing on SDS-polyacrylamide gels and Western blots

Specifications

• Content And Storage: Upon receipt store at -20°C. Product is shipped with an ice pack.
• Number of Markers: 8
• Product Type: Protein Ladder
• Ready to Load: Yes
• Recommended Applications: Tris-acetate gels
• Size Range: 60 to 250 kDa
• Stain Type: Unstained
• Gel Compatibility: Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
• Molecular Weight (g/mol): 250, 200, 150, 120, 100, 85, 70, 60 kDa
• Product Line: PageRuler™
• Quantity: 2 x 250 μL
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• System Type: Western Blotting, SDS-PAGE

For Research Use Only. Not for use in diagnostic procedures.