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Thermo Scientific™ PageRuler™ Unstained Low Range Protein Ladder

A mixture of 8 purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis.

Supplier:  Thermo Scientific™ 26632

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Catalog No. PI26632


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Description

Description

Thermo Scientific™ PageRuler Unstained Low Range Protein Ladder is a mixture of eight (8) purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis.

The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 100kDa protein band indicates the approximate position of large proteins and the 25kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins (except for the 5 and 3.4kDa peptides) contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained Broad Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Highlights:

  • Size range – seven proteins and one peptide spanning 3.4 to 100kDa

  • Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

  • Sharp bands – excellent accuracy for detection in with coomassie or silver stains

  • Reference bands – 25kDa band is stronger than the others for easy orientation

  • Tagged – each protein in the ladder contains an integral Strep-tag™ II Sequence

  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

Includes:

Proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN3, 0.01% bromophenol blue and 33% glycerol.

Recommended for:

Accurate protein sizing on SDS-polyacrylamide gels and Western blots

Specifications

Specifications

Contents: two vials of 250 µL each

Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 100 mM DTT, 1 mM NaN3, 0.01% bromophenol blue and 33% glycerol

Storage: Upon receipt store at -20°C

Protein Ladder
Tricine gels
Unstained
100, 30, 25, 20, 15, 10, 5, 3.4 kDa
2 x 250 mL
Western Blotting, SDS-PAGE
8
Yes
3.4 to 100 kDa
Bolt™ Bis-Tris Plus Gels, Novex™ Tricine Gels, Novex™ Tris-Glycine Gels, NuPAGE™ Bis-Tris Gels, NuPAGE™ Tris-Acetate Gels, SDS-PAGE Gels
PageRuler™
Approved for shipment on Wet or Dry Ice
SDS
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