Invitrogen™ Human PAK4, GST Tag Recombinant Protein

Catalog No.	Quantity
PV4212	10 μg

Catalog No. PV4212 Supplier Invitrogen™ Supplier No. PV4212

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Catalytic.

PAK4 is a serine/ threonine p21 activating kinase implicated in the reorganization of actin cytoskeleton and in the formation of filopodia. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. The second group includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.

Specifications

• Accession Number: O96013
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris with 2mM DTT, 50% glycerol, 0.5mM EDTA, 150mM NaCl, 0.02% Triton X-100 and no preservative; pH 7.5
• Gene ID (Entrez): 10298
• Molecular Weight (g/mol): 91.5 kDa
• Name: Human PAK4, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 5730488L07Rik; AW555722; hypothetical protein LOC431769; im:5916170; Kiaa1142; mKIAA1142; p21 (CDKN1A)-activated kinase 4; p21 (RAC1) activated kinase 4; p21 protein (Cdc42/Rac)-activated kinase 4; p21(CDKN1A)-activated kinase 4; p21-activated kinase 4; Pak4; PAK-4; protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs; serine/threonine-protein kinase PAK 4; wu:fd12b01; zgc:92014
• Common Name: PAK4
• Gene Symbol: Pak4
• Kinase Family: STE20 Kinase Family
• Protein Length: aa 295-591
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 295-591
• Content And Storage: Storage Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% Triton X-100, 2 mM DTT, 50% Glycerol
  Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical binding/kinetic
• Kinase Group: Ser⁄Thr Kinases: STE Group
• Shipping Condition: Dry Ice
• System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Zin-LYTE™, Omnia™ Bulk Substrate
• Target Entry: PAK4
• Target Gene: p21 protein (Cdc42⁄Rac)-activated kinase 4
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Catalytic
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Drug Discovery: Signal Transduction
• Purity or Quality Grade: 90% by SDS-PAGE