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ILEX LIFE SCIENCES LLC PAN Biotech Dispase II Neutral Protease Solution in DPBS 100 ML 1/EA
Supplier: ILEX LIFE SCIENCES LLC P10032100
PAN-Biotech Dispase II (Neutral Protease Grade II) from Bacillus polymyxa in DPBS w/o Ca Mg 2 4 U/ml 100 ml DESCRIPTION Dispase (purified neutral protease) is a non-mammalian animal origin-free neutral metalloprotease produced by Bacillus polymyxa Dispase hydrolyses N-terminal peptide bonds of non-polar amino acid residues and is classified as an aminoendopeptidase Its mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) in cell culture since it is gentle on cell membranes This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications commonly used with collagenase APPLICATION Dispase II is commonly used to separate skin epidermis from dermis (leaving intact epithelial sheets) and stem cell hepatocyte and other cell isolation applications NOTE Exact isolation conditions should be determined empirically for each cell/tissue application
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