ILEX LIFE SCIENCES LLC Immuchrom Pancreatic Elastase ELISA Kit for Human Stool Samples, 96 wells

Supplier:  ILEX LIFE SCIENCES LLC IC7400

The Immuchrom Pancreatic Elastase ELISA Kit is an immunoassay that enables easy, rapid and precise quantitative determination of pancreatic elastase in human stool samples. Calibration Range: 24.4 - 660 µg/ml. Detection Limit: 1.25 µg/ml. Principle: The pancreatic elastase test determines human pancreatic elastase according to the sandwich-principle. The elastase in sample, standard and controls binds to antibodies, which are coated to the microtiter plate. After a washing step a peroxidase labeled detection antibody is added. A second washing step is followed by the addition of the substrate which is converted to a colored product by the peroxidase. The reaction is terminated by adding an acidic stop solution. The optical densities are read at 450 nm (against the reference wavelength 620 nm) in a microtiter plate reader. The pancreatic-elastase concentration can be calculated from the standard curve. Synonyms: Pancreatic elastase-1, E1 enzyme, pancreatic endopeptidase E.

Catalog No. 50-212-0008