AdipoGen PARP-1 E988K Mutant Ctrl

Supplier:  AdipoGen AG40T0015C010

Protein. Human full-length inactive mutant E998K of PARP-1 is fused to a HA-tag and a His-tag. Source Sf21 cells. Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 50mM imidazole, 0.2% NP-40 and 10% glycerol. Purity >95% SDS-PAGE. PARP-1 ARTD1 is involved in the base excision repair BER pathway, by catalyzing the polyADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 Th1 cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

Catalog No. 50-256-2796