The Passage Reagent System (PRS) consists of a matched set of reagents optimized for releasing cells from culture for subculture or freezing, plus Attachment Factor (4x100mL). This PRS includes PRG-1 (EDTA-dPBS Solution), PRG-2 (Trypsin/EDTA-dPBS Solution), PRG-3 (Trypsin Inhibitor-dPBS Solution) and Attachment Factor. The PRS consists of PRG-1 (EDTA-dPBS Solution), PRG-2 (Trypsin/EDTA-dPBS Solution) and PRG-3 (Trypsin Inhibitor-dPBS Solution). The EDTA in PRG-1 loosens cell attachment gently from culture surfaces. Trypsin concentration in PRG-2 is optimized to detach loosened cells while minimizing adverse effects on cell viability and surface markers. Trypsin inhibitor in PRG-3 promptly halts trypsin activity once cells have been successfully released. Attachment factor promotes cell attachment. Bulk order? Contact your Fisher Scientific sales rep. Available for next day delivery after the purchase order is received. Try Our Primary Human Cells: ideal for use with media & reagents.