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ATCC pBAD24 in E. coli
Supplier: ATCC 87399
pBAD24 phagemid in Escherichia coli DH5 alpha One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. Vectors differ in replicon, antibiotic resistance marker, multiple cloning site and need for translation initiation sequences. Cultures should be grown in minimal media for more reproducible induction of expression. Expression is induced in glycerol-containing media by addition of arabinose. Expression is repressed by addition of glucose or other catabolites. Vector contains an optimized Shine-Dalgarno ribosome binding site, translation initiation codon and Kozak sequence (relevant for expression in eukaryotic cells) which promote a high level of expression of cloned inserts. The following primers can be used for sequencing of cloned inserts: 5' primer (27 - 45 bp upstream of the NheI site) 5' - CTGTTTCTCCATACCCGTT-3';
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