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Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

Manufacturer:  Gibco™ A14697

Catalog No. A14697


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Description

Description

  • Used as a Geneticin™ selectable expression plasmid for engineering stable cell lines
  • Full length human cytomegalovirus (CMV) immediate-early promoter/enhancer for high level gene expression in a wide range of mammalian cells
  • WPRE (woodchuck posttranscriptional regulatory element) downstream of the cloning site to enhance transcript expression
  • TOPO™ cloning site for rapid and efficient (>85%) cloning of Taq-amplified PCR products
  • Neomycin resistance gene for selection of stable cell lines with Geneticin™
  • pUC origin for high copy replication and maintenance of the plasmid in E. coli

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Specifications

Specifications

Constitutive
Constitutive Expression, Protein Production
CMV
1 Kit
Untagged
Transfection
Antibody and Cell Culture Production, Protein Expression, Transfection
Geneticin™ (G-418)
pcDNA
Documents
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For Research Use Only. Not for use in diagnostic procedures.