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Sigma Aldrich Fine Chemicals Biosciences PCR Buffer Without MgCl2 1
Supplier: Sigma Aldrich Fine Chemicals Biosciences 11699105001
The PCR buffer without MgCl2 in combination with the MgCl2 stock SOLUTIONon is used for the individual adjustment of the Mg2 concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200muM each. In many cases however it is required to titrate the optimal Mg2 concentration to increase the specificity and yield of the PCR reaction.
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