ACROBiosystems ACROBiosystems PE-Labeled Human PD-1 / PDCD1 Protein, Fc Tag, His Tag (recommended for neutralizing assay)

Supplier:  ACROBiosystems PD1HP2F2250TESTS

Recombinant Protein;250TESTS;PE-Labeled Human PD-1, Fc tag, His tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) is produced via conjugation of PE to Human PD-1, Fc tag, His tag under optimal conditions. Human PD-1, Fc tag, His tag is expressed in HEK293 cells containing AA Leu 25 - Gln 167 (Accession # Q15116-1).;This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 44.7 kDa. ;Flow Cytometry assay shows that PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) can bind to 293T cells overexpressing human PD-L1. 1 μl stock solution per million cells (QC tested).;FACS analysis shows that the binding of PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) to 293T overexpressing PD-L1 was inhibited by increasing concentration of neutralizing anti-human PD-L1 antibody. The IC50 is 0.29 μg/ml (QC tested).

Catalog No. 50-195-7033