Recombinant Protein;100UG;Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) (PD1-H52A3) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).;This protein carries a mouse IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.6 kDa. As a result of glycosylation, the protein migrates as 65-85 kDa under reducing (R) condition, and 130-170 kDa under non-reducing (NR) condition (SDS-PAGE).;Immobilized Human PD-1, Tag Free (Cat. No. PD1-HC214) at 0.2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Nivolumab and 1 μg/mL (100 μL/well) Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H52A3) with a half maximal inhibitory concentration (IC50) of 0.30 μg/mL (QC tested).;Immobilized Human PD-1, Tag Free, Full Length (Cat. No. PD1-HC214) at 0.2 μg/mL (100 μL/well) can bind Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H52A3) with a linear range of