Invitrogen™ Human PDGFRA [D842V], GST Tag Recombinant Protein

Catalog No.	Quantity
PV4203	10 μg

Catalog No. PV4203 Supplier Invitrogen™ Supplier No. PV4203

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Cytoplasmic.

PDGFRA is a cell surface receptor tyrosine kinase which binds members of the platelet-derived growth factor family. PDGFRA D842V is expressed in a high percentage of epithelial ovarian cancers and is implicated in the pathogenesis of acute myelogenous leukemia. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

Specifications

• Accession Number: NP_006197, D842V
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris with 0.5mM EDTA, 150mM NaCl, 0.02% Triton X-100, 50% glycerol, 2mM DTT and no preservative; pH 7.5
• Gene ID (Entrez): 114294, 5156
• Molecular Weight (g/mol): 89.7 kDa
• Name: Human PDGFRA [D842V], GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: PDGFRA D842V, PDGFR alpha
• Common Name: PDGFRA (D842V Mutant)
• Kinase Family: PDGFR Kinase Family
• Protein Length: aa 550-1089; D842V
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 550-1089; D842V
• Content And Storage: Storage Buffer: 50 mM Tris pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.02% Triton X-100, 2 mM DTT, 50% Glycerol
  Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical europium phospho-specific activity, Biochemical binding/kinetic
• Kinase Group: Tyrosine Kinases
• Shipping Condition: Dry Ice
• System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Zin-LYTE™, Omnia™ Bulk Substrate
• Target Entry: PDGFRA D842V
• Target Gene: platelet-derived growth factor receptor, alpha polypeptide
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Catalytic Domain, Cytoplasmic, Mutant
• Form: Liquid
• Protein Subtype: Receptor Tyrosine Kinases
• Research Category: Drug Discovery: Oncology, Signal Transduction
• Purity or Quality Grade: 80% by SDS-PAGE