PE Mouse Anti-Human IgA1, BD Pharmingen™

Catalog No.	Quantity
BDB571775	25 Tests
BDB571774	100 Tests

Catalog No. BDB571775 Supplier BD Biosciences Supplier No. 571775

Description

Mouse Monoclonal Secondary Antibody

Monoclonal Antibody Specifically detects in Human Samples. It is validated for Flow Cytometry.

The B3506B4 monoclonal antibody specifically recognizes the Fc region of the human Immunoglobulin A (IgA) subclass known as IgA1. IgA1 is comprised of two identical heavy chains encoded by IGHA1, and two light chains, either Igκ or Igλ, that are linked together by disulfide bonds. The B3506B4 antibody does not crossreact with other human immunoglobulin heavy chain (IgH) subclasses, including the IgA2 subclass. IgA1 is expressed by plasmablasts, plasma cells, and memory B cells as well as by some myeloma or plasmacytoma cells. IgA1 is expressed in either a transmembrane form that serves as an antigen-specific B cell surface receptor or in a soluble secreted form. Monomeric and dimeric forms of IgA1 are found in the serum although the monomeric form predominates. Multimeric forms of IgA1 connected by a J-chain and secretory component are also found in bodily secretions. Secretory IgA is transported across epithelial cells and secreted into the lumen of mucosal tissues including the gastrointestinal and respiratory tracts and is found in human breast milk. IgA1 functions in the agglutination of microbes and can block their adherence to or infection of human cells. It can also bind to CD89 (FcαRI) expressed on neutrophils, monocytes, macrophages, and eosinophils to trigger a variety of immune responses including phagocytosis, antibody-dependent cell-mediated cytotoxicity, and the release of inflammatory cytokines and mediators such as in the response to microbes.

Specifications

• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: B3506B4 (also known as DLDB4)
• Conjugate: PE
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Gene Alias: IGHA1; Immunoglobulin alpha 1
• Host Species: Mouse
• Immunogen: Human IgA1 Myeloma Protein
• Quantity: 25 Tests
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Human
• Content And Storage: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ