Gibco™ PeproGMP™ Human BDNF Recombinant Protein, PeproTech®

Catalog No. 50-274-8526

Supplier: Gibco™ GMP4500225UG

Description

Recombinant Protein

Recombinant Human BDNF is a 27.0 kDa homodimer of two 119 amino acid subunits (monomer is ~13.5 kDa) linked by strong non-covalent interactions. The BDNF recombinant protein is commonly used for neuronal differentiation in neural stem cell therapy to support regenerative medicine related to central nervous system and peripheral nervous system diseases and disorders. Reconstitute using sterile water (i.e., WFI) to a concentration of 0.1-1.0 mg/ml. Additional product information, specifications, handling and reconstitution information are provided on the Certificate of Analysis. All PeproGMP™ products are manufactured using animal-derived component free materials. Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter <1043> Ancillary Materials for Cell, Gene, and Tissue-Engineered Products.

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

Specifications

• Accession Number: P23560
• For Use With (Application): Control, Western Blot Control, Bioactivity
• Formulation: Protein with no preservative
• Gene ID (Entrez): 627
• Molecular Weight (g/mol): 13.5(monomer) kDa
• Name: PeproGMP™ Human BDNF
• Purification Method: Purified
• Quantity: 25 μg
• Storage Requirements: -20°C
• Regulatory Status: GMP
• Endotoxin Concentration: ≤0.1 EU/μg
• Gene Alias: abrineurin; ANON2; anorexia BDNF; Bdnf; BDNF precursor form; bdnf protein; brain derived neurothrophic factor; brain derived neurotrophic factor; brain-derived neurotrophic factor; BULN2; H-BDNF; neurotrophin; ProBDNF
• Common Name: BDNF
• Gene Symbol: BDNF
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Sequence: HSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNM GYTKEGCRGI DKRHWNSQCR TTQSYVRALT MDSKKRIGWR FIRIDTSCVC TLTIKRGR
• Activity: Determined by the proliferation of rat C6 cells.
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.