Gibco™ PeproGMP® Human VEGF-165 Recombinant Protein, PeproTech®

Catalog No.	Quantity
50-255-8490	100 μg
50-255-8489	1 mg
01-672-621	50 μg

Catalog No. 50-255-8490 Supplier Gibco™ Supplier No. GMP10020100UG

Description

Recombinant Protein

Recombinant Human VEGF 165 is a 38.2 kDa, disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains. Additional product information and specifications are provided on the Certificate of Analysis. All PeproGMP® products are manufactured using animal-derived component free materials. Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter 1043 Ancillary Materials for Cell, Gene, and Tissue-Engineered Products. Product use statement: For research use or further manufacturing. Not for diagnostic use or direct administration into humans or animals.

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

Specifications

• Accession Number: P15692
• For Use With (Application): Control, Western Blot Control, Bioactivity
• Format: Lyophilized
• Formulation: Protein with no preservative
• Gene ID (Entrez): 7422
• Molecular Weight (g/mol): 38.2 kDa
• Name: PeproGMP™ Human VEGF-165
• Purification Method: Purified
• Quantity: 100 μg
• Storage Requirements: -20°C or -80°C if preferred
• Regulatory Status: GMP
• Endotoxin Concentration: ≤0.1 EU/μg
• Gene Alias: H-VEGF-165; MVCD1; M-VEGF-165; vascular endothelial growth factor 165b; vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular permeability factor; VEGF; VEGF164; VEGF165; Vegfa; VEGF-A; VPF
• Common Name: VEGF-165
• Gene Symbol: Vegfa
• Biological Activity: Determined by the proliferation of HUVECs.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
• Content And Storage: -20°Cor to +80°C if preferred
• Activity: Determined by the proliferation of HUVECs.
• Expression System: E. coli
• Form: Lyophilized
• Product Line: PeproGMP
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.