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Thermo Scientific™ Pierce™ NEM (N-ethylmaleimide)

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.

Supplier:  Thermo Scientific™ 23030

Catalog No. PI23030


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Description

Description

Thermo Scientific™ Pierce N-Ethylmaleimide (NEM) is a small compound that forms stable, covalent thioether bonds with sulfhydryls (e.g., reduced cysteines), enabling them to be permanently blocked to prevent disulfide bond formation.

Highlights:

  • Forms covalent bonds with sulfhydryl groups at pH 6.5-7.5
  • Reaction can be monitored by measuring the decrease in absorbance at 300nm
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Thiol
Unreactive Group
Pierce™
Medium (10 to 30 Å)
25 g
NEM
Upon receipt store at -20°C.
Blocking Groups
Maleimide
Solid
Water
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For Research Use Only. Not for use in diagnostic procedures.