Invitrogen™ Mouse Periostin (Postn) ELISA Kit

Catalog No.	Quantity
EMPOSTNX10	10 x 96 Tests

Catalog No. EMPOSTNX10 Supplier Invitrogen™ Supplier No. EMPOSTNX10

Description

ELISA

Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor beta 1(TGF beta 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.

Specifications

• Accession Number: Q62009
• Assay Range: 0.66 to 16 ng/mL
• Assay Sensitivity: 0.05 ng/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 50706
• Gene Symbol: Postn
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Target: Periostin
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins