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Invitrogen™ PGFM Enzyme Competitive ELISA Kit

Description
The PGFM Enzyme ELISA quantitates PGFM in serum,plasma,urine,dried fecal extracts,or cell culture medium.
Principle of the method
The PGFM Competitive ELISA research-use-only kit designed to quantitatively measure PGFM present in fecal extracts,urine,serum,and plasma samples. A PGFM standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit IgG. A PGFM-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody highly specific to PGFM to each well. After a 1-hour incubation,the plate is washed and substrate is added. The substrate reacts with the bound PGFM-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Specifications
Specifications
Assay Range | 50 to 3200 pg/mL |
Assay Sensitivity | 20.8 pg/mL |
Description | PGFM Competitive ELISA Kit |
Assay | Multiplex assay on the Luminex Platform |
Conjugate | HRP |
Detection Method | Fluorescence |
Product Type | ELISA |
Protein Family | Prostaglandin |
Sample Type | Fecal Extract, Plasma, Serum, Supernatant, Urine |
Sufficient For | 96 assays |
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