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ATCC pGIKS BioB5 phagemid; in E. coli
Supplier: ATCC 87825
Nucleotides 2033 to 2341 of the gene for biotin synthetase (BioB) protein from Genbank X52327 were directionally cloned from E. coli genomic DNA into the Xho I - Not I (5`-3`) regions of the pBluescript II KS+ phagemid. The phagemid allows for either the production of an antisense transcript from the T7 promoter or a sense transcript from the T3 promoter. A 350 nucleotide transcript is produced from the T7 promoter when the construct is linearized with Xho I. Transcripts from the phagemid construct can be used for normalization and control hybridizations during microarray analyses.
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