Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. NC3553097

System Biosciences LLC pGreenFire1-ISRE (EF1α-neo) LentivectorpGreenFire1-ISRE (virus) + EF1α-Neo = >2 x 10^6 IFUs
SDP

Catalog No. NC3553097
Encompass_Preferred

Study ISRE signal transduction by co-expressing dscGFP and luciferase in response to ISRE activity (includes EF1α-neo selection cassette). With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-ISRE (EF1α-neo) Lentivector co-expresses a destabilized copepod GFP (dscGFP, 2-hour half-life) and luciferase from ISRE transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of ISRE-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to ISRE activity. The result is the ability to quantitatively measure ISRE activity by fluorescence and luciferase activity.

Catalog No. NC3553097 Supplier System Biosciences LLC Supplier No. TR016VA1
May include imposed supplier surcharges.
Add to Cart
Add to Cart

missing translation for 'validMainframeMsg'

Study ISRE signal transduction by co-expressing dscGFP and luciferase in response to ISRE activity (includes EF1α-neo selection cassette). With SBI’s line of pGreenFire1 Pathway Reporters, you can monitor signal transduction in real time. These vectors leverage our reliable lentivector technology and save you time—our pre-built signal transduction pathway reporters come as ready-to-package lentivector plasmid and ready-to-transduce pre-packaged lentivirus*. The pGreenFire1-ISRE (EF1α-neo) Lentivector co-expresses a destabilized copepod GFP (dscGFP, 2-hour half-life) and luciferase from ISRE transcriptional response elements (TREs) paired with a minimal CMV promoter (mCMV). The mCMV promoter alone delivers negligible expression, but when downstream of ISRE-responsive transcriptional elements, drives expression of dscGFP and luciferase in response to ISRE activity. The result is the ability to quantitatively measure ISRE activity by fluorescence and luciferase activity.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.