Thermo Scientific™ pH Gradient Buffers

Catalog Number	Mfr. No.	Description	pH	Quantity
DX083273	Thermo Scientific™ 083273	CX-1 pH Gradient Buffer A	5.6	125 mL
DX083275	Thermo Scientific™ 083275	CX-1 pH Gradient Buffer B	10.2	125 mL
DX085346	Thermo Scientific™ 085346	CX-1 pH Gradient Buffer A	5.6	250 mL
DX085348	Thermo Scientific™ 085348	CX-1 pH Gradient Buffer B	10.2	250 mL
DX302779	Thermo Scientific™ 302779	CX-1 pH Gradient Buffer A	5.6	500 mL
DX302780	Thermo Scientific™ 302780	CX-1 pH Gradient Buffer B	10.2	500 mL
03-254-083	Thermo Scientific™ 303275	CX-1 pH Gradient Buffer B	10.2	1 L
DX085349	Thermo Scientific™ 085349	CX-1 pH Gradient Buffer Kit; 250 mL Buffer A + 250 mL Buffer B	5.6 to 10.2	250 mL + 250 mL

Description

Obtain fast and highly robust, reproducible HPLC gradients using Thermo Scientific™ CX-1 pH Gradient Buffers. Simple to optimize and easily automated, these buffers are applicable for HPLC separation of the majority of monoclonal antibody (mAb) biotherapeutics drugs. CX-1 pH Gradient Buffers are ready to use with existing HPLC columns without the need to formulate mobile phases. The CX-1 buffers save time in method development, facilitate method transfer to QA/QC for a wide range of mAb charge variants through a generic HPLC-based approach to mAb characterization, and do not require time-consuming mobile phase adjustments.

pH buffer solutions can be used to generate highly reproducible, linear pH gradients in cation exchange chromatography.

• Robust, reproducible gradients
• Predict biomolecule pH
• Simple method optimization
• Easily automated
• Applicable to the majority of MAbs

Unlike traditional cation exchange chromatography using salt gradients, it is possible to predict the pH and the expected retention of the charge variants and use a narrow pH range to get a higher resolution separation.

Specifications

• Product Line: Thermo Scientific