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Sigma Aldrich Fine Chemicals Biosciences PhasePrepTM BAC DNA Kit Sc
Supplier: Sigma Aldrich Fine Chemicals Biosciences NA01001KT
The PhasePrep(TM) BAC DNA Kit offers a scalable and cost-effective method for isolating large-molecular weight plasmids such as Bacterial Artificial Chromosomes (BAC) from recombinant E. coli cultures. The same kit can be used for preparations of four different sizes sufficient reagents are provided for 300 micro 180 mini 30 midi or 15 maxi preparations. Up to 2 12 50 or 100 mg of BAC DNA can be recovered from 5 40 250 or 500 ml of overnight recombinant E. coli culture respectively. The purified BAC DNA contains very low levels of endotoxin (A£10 EU/mg DNA).Recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure. Nucleic acid is precipitated from the cleared lysate residual RNA is removed by a short digestion at elevated temperature with an RNase cocktail. Endotoxins and other impurities are removed by simple temperature-mediated extraction and phase separation. finally the BAC DNA is selectively precipitated from sol
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